|标题: Inhibition of multidrug-resistant foodborne Staphylococcus aureus biofilms by a natural terpenoid (+)-nootkatone and related molecular mechanism|
|作者: Farha, AK (Farha, Arakkaveettil Kabeer); Yang, QQ (Yang, Qiong-Qiong); Kim, G (Kim, Gowoon); Zhang, D (Zhang, Dan); Mavumengwana, V (Mavumengwana, Vuyo); Habimana, O (Habimana, Olivier); Li, HB (Li, Hua-Bin); Corke, H (Corke, Harold); Gan, RY (Gan, Ren-You)|
|来源出版物: FOOD CONTROL 卷: 112 文献号: 107154 DOI: 10.1016/j.foodcont.2020.107154 出版年: JUN 2020 |
|影响因子 (2018 年): 4.248|
|摘要: Staphylococcus aureus, a foodbome pathogen, poses serious problems to the food industries owing to biofilm formation, and over 25% of the foodbome illnesses in China have been attributed to S. aureus only. Phytochemicals are widely used as anti-biofilm agents with promising efficacy, and most of them are widely available and safe. This study reported the anti-biofilm efficacy of (+)-nootkatone, a sesquiterpene ketone found in a common fruit grapefruit, against multidrug-resistant S. aureus and its potential molecular mechanism. (+)-Nootkatone exhibited bacteriostatic and bactericidal effects at 200 and 400 mu g/mL, respectively, against S. aureus SJTUF 20758 and S. aureus ATCC 25923. Crystal violet staining indicated that (+)-nootkatone inhibited S. aureus biofilm formation (p < 0.05) at a sub-MIC of 50 mu g/mL and reduced exopolysaccharide production. The thickness of biofilms was significantly reduced by (+)-nootkatone, which was supported by the light microscopy and confocal laser scanning microscopy. Growth curve of bacteria showed that the antibacerial activity of (+)-nootkatone was dose-dependent, and the sub-MIC concentrations did not affect the bacterial growth of planktonic cells. Besides, (+)-nootkatone affected the sliding motility of S. aureus. At 200 mu g/mL, (+)-nootkatone led to the reduction of preformed biofilm mass by 50% and bacterial cell death of 79%, accompanied with a reduction of exopolysaccharide. The expression of biofilm-related genes, including sarA, icaA, agrA, RNAIII, and spa, was suppressed by (+)-nootkatone, as revealed by the transcriptional analysis. Additionally, MTT assay revealed that there was no toxicity of (+)-nootkatone to the human foreskin fibroblasts (HFF) cells. Therefore, (+)-nootkatone is a promising phytochemical against S. aureus biofilms, and has the potential to be used in food industry to fight against S. aureus-induced safety issues.|
|作者关键词: (+)-Nootkatone; Phytochemical; Multidrug resistance; Staphylococcus aureus; Biofilm; Mechanisms|
|KeyWords Plus: ANTIBIOFILM ACTIVITY; GALLIC ACID; EPIDERMIDIS; PREVALENCE; EXPRESSION; NOOTKATONE; ASSAYS; SARA; ICA|
|地址: [Farha, Arakkaveettil Kabeer; Yang, Qiong-Qiong; Kim, Gowoon; Zhang, Dan; Corke, Harold; Gan, Ren-You] Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Food Sci & Technol, Shanghai 200240, Peoples R China.|
[Mavumengwana, Vuyo] Stellenbosch Univ, Fac Med & Hlth Sci, Div Mol Biol & Human Genet,US SAMRC Ctr TB Res, Dept Biomed Sci,DST NRF Ctr Excellence Biomed TB, ZA-8000 Cape Town, South Africa.
[Habimana, Olivier] Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China.
[Li, Hua-Bin] Sun Yat Sen Univ, Sch Publ Hlth, Dept Nutr, Guangdong Prov Key Lab Food Nutr & Hlth, Guangzhou 510080, Peoples R China.
[Gan, Ren-You] Chinese Acad Agr Sci, Inst Urban Agr, Res Ctr Plants & Human Hlth, Chengdu 610213, Peoples R China.
|通讯作者地址: Corke, H; Gan, RY (通讯作者)，Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Food Sci & Technol, Shanghai 200240, Peoples R China.|
Gan, RY (通讯作者)，Chinese Acad Agr Sci, Inst Urban Agr, Res Ctr Plants & Human Hlth, Chengdu 610213, Peoples R China.
|电子邮件地址: firstname.lastname@example.org; email@example.com|
|作者Web of Science ResearcherIDORCID 号Habimana, Olivier 0000-0002-6357-4285 Mavumengwana, Vuyo 0000-0003-4672-6176 Gan, Ren-You 0000-0002-4162-1511 |
|出版商: ELSEVIER SCI LTD|
|出版商地址: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND|
|Web of Science 类别: Food Science & Technology|
|研究方向: Food Science & Technology|
|IDS 号: KU4AP|
|29 字符的来源出版物名称缩写: FOOD CONTROL|
|ISO 来源出版物缩写: Food Control|
|基金资助机构授权号National Key R&D Program of China 2017YFC1600100 China Central Public-interest Scientific Institution Basal Research Fund Y2020XK05 Shanghai Basic and Key Program 18JC1410800 Shanghai Pujiang Talent Plan 18PJ1404600 |
This work was supported by the National Key R&D Program of China (Grant No. 2017YFC1600100), China Central Public-interest Scientific Institution Basal Research Fund (Grant No.Y2020XK05), the Shanghai Basic and Key Program (Grant No. 18JC1410800), and the Shanghai Pujiang Talent Plan (Grant No. 18PJ1404600).