Guo Huan, Feng Kang-Lin, Zhou Jia, Liu Lu, Wei Si-Yu, Zhao Li, Qin Wen, Gan Ren-
来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
|标题: Carboxymethylation of Qingke beta-glucans and their physicochemical properties and biological activities|
|作者: Guo, H (Guo, Huan); Feng, KL (Feng, Kang-Lin); Zhou, J (Zhou, Jia); Liu, L (Liu, Lu); Wei, SY (Wei, Si-Yu); Zhao, L (Zhao, Li); Qin, W (Qin, Wen); Gan, RY (Gan, Ren-You); Wu, DT (Wu, Ding-Tao)|
|来源出版物: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 卷: 147 页: 200-208 DOI: 10.1016/j.ijbiomac.2020.01.050 出版年: MAR 15 2020 |
|影响因子 (2018 年): 4.784|
|摘要: In this study, the response surface methodology was used to optimize the reaction conditions of carboxymethylated modification of Qingke beta-glucans (QG), and effects of different degrees of carboxymethylation (high, medium, and low) on their structural characteristics, in vitro antioxidant activities, and in vitro hypolipidemic activities were studied. The optimal reaction conditions of carboxymethylated Qingke beta-glucans (QG-Cs) with high degree of carboxymethylation (DC = 0.90) were as follows: reaction temperature of 62.1 degrees C, reaction time of 329 h, and concentration of chloroacetic add of 1.75 mol/L. Results demonstrated that the carboxymethylated modification significantly affected the solubilities, molar ratios of constituent monosaccharides, molecular weights, and apparent viscosities of QG. Indeed, the QG-Cs exhibited much higher antioxidant activities (reducing powers, NO, and DPPH radical scavenging activities), in vitro binding properties (fat, bile acid, and cholesterol binding capacities), and pancreatic lipase inhibition activities than that of QG. Furthermore, results showed that bioactivities of QG-Cs were closely correlated to their carboxymethyl groups. Results suggested that the carboxymethylated modification could be an efficient method for enhancing bioactivities of QG, and QG-Cs had good potential applications in bio-pharmaceutical industry. (C) 2020 Elsevier B.V. All rights reserved.|
|PubMed ID: 31923502|
|作者关键词: Hordeum vulgare L.; Carboxymethylated modification; Structural characteristics; Antioxidant activity; Hypolipidemic activity|
|KeyWords Plus: IN-VITRO ANTIOXIDANT; RHEOLOGICAL PROPERTIES; EXTRACTION METHODS; POLYSACCHARIDES; CAPACITIES|
|地址: [Guo, Huan; Feng, Kang-Lin; Zhou, Jia; Liu, Lu; Wei, Si-Yu; Zhao, Li; Qin, Wen; Wu, Ding-Tao] Sichuan Agr Univ, Coll Food Sci, Inst Food Proc & Safety, Yaan 625014, Sichuan, Peoples R China.|
[Gan, Ren-You] Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610213, Peoples R China.
|通讯作者地址: Wu, DT (通讯作者)，Sichuan Agr Univ, Coll Food Sci, Inst Food Proc & Safety, Yaan 625014, Sichuan, Peoples R China.|
Gan, RY (通讯作者)，Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610213, Peoples R China.
|电子邮件地址: firstname.lastname@example.org; DT_Wu@sicau.edu.cn|
|作者Web of Science ResearcherIDORCID 号Gan, Ren-You 0000-0002-4162-1511 |
|出版商地址: RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS|
|Web of Science 类别: Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science|
|研究方向: Biochemistry & Molecular Biology; Chemistry; Polymer Science|
|IDS 号: LD2LQ|
|29 字符的来源出版物名称缩写: INT J BIOL MACROMOL|
|ISO 来源出版物缩写: Int. J. Biol. Macromol.|
|基金资助机构授权号Scientific Research Foundation of Sichuan Agricultural University 03120321 Scientific Research Fund Project of Department of Science and Technology of Sichuan Province 2018JY0149 National Natural Science Foundation of China 31901690 Technology Innovation Programof Chinese Academy of Agricultural Sciences |
Thisworkwas supported by the Scientific Research Foundation of Sichuan Agricultural University (grant number 03120321), the Scientific Research Fund Project of Department of Science and Technology of Sichuan Province (grant number 2018JY0149), the National Natural Science Foundation of China (grant number 31901690), and the Technology Innovation Programof Chinese Academy of Agricultural Sciences (grant number ASTIP).