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Xiong Fangjie, Liu Mei, Zhuo Fengping, Yin Huan, Deng Kexuan, Feng Shun, Liu Yud

来源:MOLECULAR PLANT PATHOLOGY 发布时间:2019-12-13 18:47:24 浏览次数:74次

标题: Host-induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould
作者: Xiong, FJ (Xiong, Fangjie); Liu, M (Liu, Mei); Zhuo, FP (Zhuo, Fengping); Yin, H (Yin, Huan); Deng, KX (Deng, Kexuan); Feng, S (Feng, Shun); Liu, YD (Liu, Yudong); Luo, XM (Luo, Xiumei); Feng, L (Feng, Li); Zhang, SM (Zhang, Shumin); Li, ZG (Li, Zhengguo); Ren, MZ (Ren, Maozhi)
来源出版物: MOLECULAR PLANT PATHOLOGY  卷: 20  期: 12  页: 1722-1739  DOI: 10.1111/mpp.12873  提前访问日期: OCT 2019   出版年: DEC 2019  
影响因子 (2018 年): 4.379

摘要: Botrytis cinerea is the causal agent of grey mould for more than 200 plant species, including economically important vegetables, fruits and crops, which leads to economic losses worldwide. Target of rapamycin (TOR) acts a master regulator to control cell growth and proliferation by integrating nutrient, energy and growth factors in eukaryotic species, but little is known about whether TOR can function as a practicable target in the control of plant fungal pathogens. Here, we characterize TOR signalling of B. cinerea in the regulation of growth and pathogenicity as well as its potential value in genetic engineering for crop protection by bioinformatics analysis, pharmacological assays, biochemistry and genetics approaches. The results show that conserved TOR signalling occurs, and a functional FK506-binding protein 12 kD (FKBP12) mediates the interaction between rapamycin and B. cinerea TOR (BcTOR). RNA sequencing (RNA-Seq) analysis revealed that BcTOR displayed conserved functions, particularly in controlling growth and metabolism. Furthermore, pathogenicity assay showed that BcTOR inhibition efficiently reduces the infection of B. cinerea in plant leaves of Arabidopsis and potato or tomato fruits. Additionally, transgenic plants expressing double-stranded RNA of BcTOR through the host-induced gene silencing method could produce abundant small RNAs targeting BcTOR, and significantly block the occurrence of grey mould in potato and tomato. Taken together, our results suggest that BcTOR is an efficient target for genetic engineering in control of grey mould, and also a potential and promising target applied in the biocontrol of plant fungal pathogens.
入藏号: WOS:000490732200001
PubMed ID: 31622007
语言: English
文献类型: Article
作者关键词: Botrytis cinerea; host-induced gene silencing; mycelial growth; pathogenicity; target of rapamycin
KeyWords Plus: KINGDOM RNA TRAFFICKING; TOR SIGNALING PATHWAY; RAPAMYCIN TOR; ENVIRONMENTAL RNAI; VERTICILLIUM WILT; KINASE; TARGET; MTOR; GROWTH; PROTEIN
地址: [Xiong, Fangjie; Liu, Mei; Zhuo, Fengping; Yin, Huan; Deng, Kexuan; Feng, Shun; Liu, Yudong; Luo, Xiumei; Feng, Li; Zhang, Shumin; Li, Zhengguo; Ren, Maozhi] Chongqing Univ, Sch Life Sci, Chongqing 401331, Peoples R China. 
[Xiong, Fangjie; Liu, Mei; Zhuo, Fengping; Yin, Huan; Deng, Kexuan; Feng, Shun; Liu, Yudong; Luo, Xiumei; Feng, Li; Zhang, Shumin; Li, Zhengguo; Ren, Maozhi] Key Lab Plant Hormone & Dev Regulat Chongqing, Chongqing 407337, Peoples R China. 
[Zhuo, Fengping] Chongqing Univ Sci & Technol, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China. 
[Ren, Maozhi] Chinese Acad Agr Sci, Inst Urban Agr, Natl Chengdu Agr Sci & Technol Ctr, Inst Urban Agr, Chengdu 610000, Sichuan, Peoples R China.
通讯作者地址: Ren, MZ (通讯作者),Chongqing Univ, Sch Life Sci, Chongqing 401331, Peoples R China.
Ren, MZ (通讯作者),Key Lab Plant Hormone & Dev Regulat Chongqing, Chongqing 407337, Peoples R China.
Ren, MZ (通讯作者),Chinese Acad Agr Sci, Inst Urban Agr, Natl Chengdu Agr Sci & Technol Ctr, Inst Urban Agr, Chengdu 610000, Sichuan, Peoples R China.
电子邮件地址: renmaozhi@cqu.edu.cn
出版商: WILEY
出版商地址: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Web of Science 类别: Plant Sciences
研究方向: Plant Sciences
IDS 号: JN4FF
ISSN: 1464-6722
eISSN: 1364-3703
29 字符的来源出版物名称缩写: MOL PLANT PATHOL
ISO 来源出版物缩写: Mol. Plant Pathol.
来源出版物页码计数: 18
基金资助致谢:
基金资助机构授权号National Natural Science Foundation of China 31801913 31972469 31672206 31801271 China Postdoctoral Science Foundation 2017M622958 2018M633320 Project of Chongqing Science and Technology Commission cstc2016jcyjA0822 cstc2019jcyj-msxmX0127 cstckjcxljrc15 Chengdu Agricultural Science and Technology Center local financial special fund project NASC2019TI13 Fundamental Research Funds for the Central Institutes  Chinese Academy of Agricultural Sciences 19-001-09 

We thank Guozheng Qin (Chinese Academy of Sciences, Beijing, China) for kindly providing B. cinerea strain B05.10. This work was supported by the following grants: the National Natural Science Foundation of China (nos. 31801913, 31972469, 31672206 and 31801271), the China Postdoctoral Science Foundation (nos. 2017M622958 and 2018M633320), the Project of Chongqing Science and Technology Commission (nos. cstc2016jcyjA0822, cstc2019jcyj-msxmX0127 and cstckjcxljrc15), Chengdu Agricultural Science and Technology Center local financial special fund project (NASC2019TI13), the Fundamental Research Funds for the Central Institutes and the Chinese Academy of Agricultural Sciences (19-001-09).

开放获取: DOAJ Gold


Host-induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould.pdf