Xiong Fangjie, Liu Mei, Zhuo Fengping, Yin Huan, Deng Kexuan, Feng Shun, Liu Yud
来源：MOLECULAR PLANT PATHOLOGY 发布时间：2019-12-13 18:47:24 浏览次数:74次
|Xiong, FJ (Xiong, Fangjie); Liu, M (Liu, Mei); Zhuo, FP (Zhuo, Fengping); Yin, H (Yin, Huan); Deng, KX (Deng, Kexuan); Feng, S (Feng, Shun); Liu, YD (Liu, Yudong); Luo, XM (Luo, Xiumei); Feng, L (Feng, Li); Zhang, SM (Zhang, Shumin); Li, ZG (Li, Zhengguo); Ren, MZ (Ren, Maozhi)|
|Botrytis cinerea is the causal agent of grey mould for more than 200 plant species, including economically important vegetables, fruits and crops, which leads to economic losses worldwide. Target of rapamycin (TOR) acts a master regulator to control cell growth and proliferation by integrating nutrient, energy and growth factors in eukaryotic species, but little is known about whether TOR can function as a practicable target in the control of plant fungal pathogens. Here, we characterize TOR signalling of B. cinerea in the regulation of growth and pathogenicity as well as its potential value in genetic engineering for crop protection by bioinformatics analysis, pharmacological assays, biochemistry and genetics approaches. The results show that conserved TOR signalling occurs, and a functional FK506-binding protein 12 kD (FKBP12) mediates the interaction between rapamycin and B. cinerea TOR (BcTOR). RNA sequencing (RNA-Seq) analysis revealed that BcTOR displayed conserved functions, particularly in controlling growth and metabolism. Furthermore, pathogenicity assay showed that BcTOR inhibition efficiently reduces the infection of B. cinerea in plant leaves of Arabidopsis and potato or tomato fruits. Additionally, transgenic plants expressing double-stranded RNA of BcTOR through the host-induced gene silencing method could produce abundant small RNAs targeting BcTOR, and significantly block the occurrence of grey mould in potato and tomato. Taken together, our results suggest that BcTOR is an efficient target for genetic engineering in control of grey mould, and also a potential and promising target applied in the biocontrol of plant fungal pathogens.|
|Botrytis cinerea; host-induced gene silencing; mycelial growth; pathogenicity; target of rapamycin|
|KINGDOM RNA TRAFFICKING; TOR SIGNALING PATHWAY; RAPAMYCIN TOR; ENVIRONMENTAL RNAI; VERTICILLIUM WILT; KINASE; TARGET; MTOR; GROWTH; PROTEIN|
|[Xiong, Fangjie; Liu, Mei; Zhuo, Fengping; Yin, Huan; Deng, Kexuan; Feng, Shun; Liu, Yudong; Luo, Xiumei; Feng, Li; Zhang, Shumin; Li, Zhengguo; Ren, Maozhi] Chongqing Univ, Sch Life Sci, Chongqing 401331, Peoples R China.|
[Xiong, Fangjie; Liu, Mei; Zhuo, Fengping; Yin, Huan; Deng, Kexuan; Feng, Shun; Liu, Yudong; Luo, Xiumei; Feng, Li; Zhang, Shumin; Li, Zhengguo; Ren, Maozhi] Key Lab Plant Hormone & Dev Regulat Chongqing, Chongqing 407337, Peoples R China.
[Zhuo, Fengping] Chongqing Univ Sci & Technol, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China.
[Ren, Maozhi] Chinese Acad Agr Sci, Inst Urban Agr, Natl Chengdu Agr Sci & Technol Ctr, Inst Urban Agr, Chengdu 610000, Sichuan, Peoples R China.
|Ren, MZ (通讯作者)，Chongqing Univ, Sch Life Sci, Chongqing 401331, Peoples R China.|
Ren, MZ (通讯作者)，Key Lab Plant Hormone & Dev Regulat Chongqing, Chongqing 407337, Peoples R China.
Ren, MZ (通讯作者)，Chinese Acad Agr Sci, Inst Urban Agr, Natl Chengdu Agr Sci & Technol Ctr, Inst Urban Agr, Chengdu 610000, Sichuan, Peoples R China.
|基金资助机构授权号National Natural Science Foundation of China 31801913 31972469 31672206 31801271 China Postdoctoral Science Foundation 2017M622958 2018M633320 Project of Chongqing Science and Technology Commission cstc2016jcyjA0822 cstc2019jcyj-msxmX0127 cstckjcxljrc15 Chengdu Agricultural Science and Technology Center local financial special fund project NASC2019TI13 Fundamental Research Funds for the Central Institutes Chinese Academy of Agricultural Sciences 19-001-09|